Analysis Note

Positive ControlHeLa cells, PC12 cells, NIH3T3 cells, Human small intestine tissue

Negative Control293T cells

Application

ELISA (see coments)Immunoblotting (1-5 g/ml)Immunocytochemistry (10 g/ml)Paraffin Sections (3 g/ml)

General description

Recognizes the ~12-16 kDa TOMM20 protein in HeLa, PC12, and NIH3T3 cells and human small intestine tissue.

Purified mouse monoclonal antibody. Recognizes the ~12-16 kDa TOMM20 protein.

This Anti-TOMM20 Mouse mAb (4F3) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of TOMM20.

Immunogen

Full-length, recombinant, human TOMM20 (aa 1-146), expressed as a GST fusion protein

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Regulatory Review (Z)